PostEra

Latest activity data missing RapidFire IC50s?

COVID
#1

I’m trying to analyze the activity latest data posted by the London lab: https://postera.ai/covid/activity_data.csv

On the https://postera.ai/covid/activity_data page, there is a nice scatter plot comparing the RapidFire pIC50 values against the fluorescence values, but the full list of ‘r_IC50’ values appears to be incomplete.

Also I think there is some problem with the values being plotting in the scatter plot. For example: take the compound PAU-UNI-8cdd41c7-1 with the Fluorescence IC50 of 1.89 µM, and RapidFire IC50 of 0.57 µM. This should have Fluorescence pIC50 of -log10(1.89e-6) = 5.72 as is shown in the table. But the scatter plot shows this data point at 4.7 on the x-axis (?)

Here’s what I get, at least from the numbers in current activity_data.csv:

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#2

Thanks @vvoelz, I’ll look into this. Part of the reason for missing rapidFire data is that not as many have been run as for fluorescence. Also, things are coming in at different times. As for why the pIC50’s are off – that is troubling and will be looked at.

#3

I just downloaded https://postera.ai/covid/activity_data.csv and it has been apparently fixed :slight_smile:

image

#4

Oh yes, sorry – forgot to let you know I fixed this errror! It was a stupid factor of 10 error – completely my fault.